The purpose of study was to compare four different vaccination routes. The study was conducted at environment controlled broiler house, University of Veterinary and Animal Sciences, Ravi campus-Pattoki. In this experiment 264 commercial day old chicks were purchased from hatchery.On arrival, 24 day-old chicks were sent to laboratory for determining the maternal antibody level and remaining 240 birds were divided randomly in to four treatments (Drinking water, Eye drop, Spray,Spray +Drinking water) groups. Each group was replicated 6 times with 10 birds each. Before vaccination 03 birds were picked up randomly from each replicate and 3 ml blood was collected from the main brachial vein to determine NDV and IBV maternal antibody titers of the chickens. At the end of the trial, 03 birds were picked up randomly, slaughtered to determine the weight of immune organs and carcass characteristics. The data thus obtained were statistically analyzed through Completely Randomized Design (CRD) using one way analysis of variance (ANOVA) techniques at 0.05 significane level. The means were compared using Duncan’s Multiple Range (DMR) test with the help of SAS, 9.1.Results of present study showed non-significant differences (P ≥ 0.05) infeed intake, body weights, feed conversion ratio, feed efficiency, point spread, performance index andproduction number. Significant differences (P ≤0.05) were observed in mortality and livability percentage. The birds vaccinated thorugh the eye drop showed higher livability than spray with drinking water.Significant differences (P ≤ 0.05) were observed inBursa and thymusweights with respect to four routes of vaccination. The birds vaccinated through eye drop method had the higher bursa and thymusweight as compared to those vaccinated through drinking water, eye drop and spray with drinking water. There were non-significant (P ≥ 0.05) in spleen weight.Maternal antibody level against Infectious Bronchitis and Newcastledisease was 3421and 6.9 respectively. Decline in maternal antibody titer was recorded onthe 7th and 15th day of the experiment.Vaccination at 17th day boosted the immune response.Significant differences (P ≤ 0.05) were observed in an immune response against the Infectious Bronchitis and Newcastle Disease. The birds vaccinated through eye drop route showed higher immune response than other routes of vaccination.Non-significant differences (P ≥ 0.05) were observed in dressing percentage, breast yield percentage, gizzard percentage, heart percentage, keel length, shank length, intestinal length and intestinal weight. Significant differences (P ≤0.05) were observed in thigh percentage.
Conclusion:
Based on the findings of the present study it may be stated that
1. Vaccination routes had no effect on growth performance.
2. Eye drop vaccinataion developed better immunity and also had higher weight of immune organs, while, spray remained poorest.
Suggestion and Recommendations:
For future it is recommended as eye drop method of vaccination is the best route of vaccination for higher immune response and higher immune organs weight and others routes of vaccinations has lower immune response and imuune organs weight. Continuous detailed and comprehensive research investigations are needed on different vaccination routes.